Grammys: Macklemore and Madonna Perform 'Same Love' As 33 Couples Wed Live on Air (Video)

The music giants joined forces to promote marriage equality as both gay and straight couples were married at the same time.

Macklemore & Ryan Lewis' "Same Love" anthem became the theme song for 33 newlyweds during the 56th Grammy Awards on Sunday night.

The gay and straight weddings were officiated by Queen Latifah while the hit song about equality was performed by the rap duo with Madonna hitting the stage with them, along with Mary Lambert and jazz artist Trombone Shorty.

PHOTOS: Grammys 2014: Red Carpet Arrivals

"This song is not a love song for some of us but for all of us," said Latifah as she introduced the special segment. 

The mass of couples exchanged rings at the same time as the singer pronounced them wed while Madonna emerged to sing her classic "Open Your Heart."

The Material Girl changed into a white Ralph Lauren suit for the ground-breaking performance after wearing a dramatic black tux on the carpet.

At the end of the historical television moment, Ringo Starr was overheard saying to Paul McCartney, "Let's all renew our vows." McCartney then shook the hands of every couple that walked by.

STORY: Grammys Beauty: Madonna and Robin Thicke's Facialist Speaks

GLAAD President Sarah Kate Ellis celebrated the event, telling The Hollywood Reporter in an exclusive statement.

"When such a critically acclaimed and popular rap artist puts marriage equality center stage at one of the biggest events of the year, it is the latest in a long line of signs that our nation not only accepts, but celebrates the love and commitment of gay couples today," said Ellis (who is married to Kristen Henderson of the band Antigone Rising).

"Macklemore and Ryan Lewis aren't only talented musicians, but are setting the bar high for our allies around the country today."

GLAAD also tweeted its support of the equal rights awareness performance earlier in the evening, saying: "Congrats to #LGBT allies @macklemore and@RyanLewis! #GRAMMYs."

When asked by Ryan Seacrest about the groundbreaking event before the show, Madonna stumbled over her words, saying: "I was told not to say anything.

VIDEO: Grammys Moment: Jay Z Tells Baby Blue Ivy, 'Daddy Got a Gold Sippy Cup for You'

"I mean I wouldn't do any old song with any old artist, but history is going to be made tonight," she finally revealed, while speaking through a gold grill. 

Among the new brides was Lewis' sister, Laura, who reportedly said "I do," to boyfriend, Alex, on live TV. It was originally announced that 34 couples would tie the knot, but Latifah stated 33 in her introduction on stage during the show.

STORY: Lorde on Her First Grammys: 'Sounds Like Prom on 'Roids

After being deputized by Los Angeles County, Latifah officiated the mass nuptials, which included "gay, straight, old, young, of many races and many colors," according to the New York Times

"Same Love," which features songwriter Lambert on the chorus, overtly addresses the cultural divide on the issue of gay marriage. "A certificate on paper isn't going to solve it all/but it's a damn good place to start," read the lyrics. The title is nominated in the song of the year category at the awards.  

Speaking to The Hollywood Reporter earlier in the week, Grammys producer Ken Ehrlich pledged a night of surprises.

"All things are possible on the Grammy Awards," the producer said when previously asked about the Madonna appearance, later adding: "It’s what we do, one of the trademarks of this show, what I’d like to believe separates the Grammys from these other shows. You see artists do things that they just don’t do anywhere else."

PHOTOS: Grammys Fashion: The 22 Best Style Moments of All Time

Macklemore & Ryan Lewis were nominated for seven honors at the 2014 awards, including album of the year for The Heist.

They were early winners for best new artist, as well as taking home Grammys for best rap album, best rap performance and best rap song for "Thrift Shop."